05 Sequencing

Unfortunately not.  The samples are put into library prep and sequencing queues after we have received both, the submission form and the sample.  Submission forms have to be submitted in hard-copy (accompanying the samples) and electronically (please email the spreadsheet forms to us).

DNA samples and sequencing libraries will be stored at -20 degrees.  RNA samples will be stored at  -70 degrees C. However samples and libraries will only be stored for one year after sample submission.  Please notify us two days in advance if you plan to pick up your samples. Please arrange the sample pick-up within two months of the data delivery.   Please provide a FedEx account number and a shipping address if your samples should be shipped back.

We carry out a lot of 16S sequencing - almost daily - with customer prepared samples.

The DNA Technologies and Expression Analysis Cores do not offer Sanger DNA Sequencing, but these services are available on campus. See the UC DNA Sequencing Facility or the CAES Genomics Facility for more information.

Work in the Core is performed on a “first come, first served” basis. HiSeq sequencing data will typically be delivered within about two to five weeks after library submission, while MiSeq has a 5-8 day turnaround time. The turnaround time can fluctuate and is dependent on the number of customer samples in the queue and the read types and lengths requested.  Similarly library prep projects will be grouped according to the requested library prep protocols to allow for efficient processing.

Certainly. Please provide a Bioanalyzer profile (we can also generate these), and barcode  sequence information in the sample submission form. We will check the quantity of your libraries using real-time PCR (included in the sequencing price). We suggest to submit your library in at least 15 ul volume at a minimum concentration of 5 nM. Please see the Sample Requirements page for details.

We generate libraries for sequencing with Illumina and PacBio instruments. Illumina libraries include genomic DNA, RNA-seq, ChIP-seq, micro RNA, small RNA, Methyl-seq, RRBS, and reduced representation libraries suitable for GBS analyses, Tag-Seq, and Tn-Seq. Please inquire with us if your protocol is not currently listed. Please see the Library Prep page and the Sample Requirements page for details.