We offer spatial biology and transcriptomics studies on the10X Genomics Xenium and Visium HD platforms.
The 10x Genomics Xenium platform provides high-resolution, subcellular spatial transcriptomics for both fresh frozen and fixed tissue sections, and animal and plant tissues. Using sensitive padlock probe and rolling circle amplification (RCA) technology, it precisely quantifies hundreds to thousands of different RNA targets, including rare or degraded transcripts and specific isoforms. These can be supplemented with protein panels. The technology involves specialized slides (with 12 x 24 mm imageable area/slide), hybridizing custom or pre-designed probe sets (up to 5,000), and running the assays on the Xenium Analyzer including automated microscopy. Over a two-day automated run, the system uses successive rounds of fluorescent imaging to map gene-specific optical signatures directly to their spatial locations within the tissue. Results are then processed and visualized through free Explorer software.
For more details and the latest options please see the manufacturer's information.
This page provides the currently available ready-to-use panels and information on the design of custom panels.
Due to the pricing of the reagents, a Xenium Analyzer run comprises two slides that will be processed together. Different panels can be run on each slide.
Xenium Sample Prep
The imageable area of the Xenium slides is outlined by a white line measuring 12 mm x 24 mm. The sample positioning area within is slightly smaller (235 mm2, 10.45 mm x 22.45 mm) and is surrounded by fiducials. Tissue sections are placed within the Sample Area, making sure they (and any surrounding wax) don't cover the fiducials or overlap each other. You can fit as many sections as the space allows. If you have multiple samples, it's best to co-embed them into a single block. Just ensure that the block is dedicated to a single panel rather than a specific tissue type.
These documents provide instructions for Xenium Add-on Panels designs (for human and mouse) and Custom Panel designs:
https://www.10xgenomics.com/support/in-situ-gene-expression/documentation/steps/panel-design