Illumina High Throughput Sequencing

Illumina sequencing platforms generate up to 930 gigabases of high-quality sequence data per lane (NovaSeq X 25B) using a massively parallel short-read sequencing approach. The Illumina systems use sequencing-by-synthesis (SBS) technology and reversible terminator chemistry. More information describing the technology and the instrument is available from Illumina here

The DNA Technologies and Expression Analysis Core at the Genome Center operate a Nextseq 500 and a MiSeq. We are also sequencing on the NovaSeq X. Steps involved in an Illumina sequencing experiment can be broken down into a series of experimental manipulations, instrument runs, and data analyses. These steps include creation of sequencing libraries, seeding clustering of the flow cell on the sequencer, sequencing by synthesis, and bioinformatics. Please also see the "Beginner's Guide to NGS" information and the video at the bottom of this page, both from Illumina.

Getting started info: > Register with the Genome Center > Sample Requirements > Sample Submission

Read Length - Data Output

Current average short-read sequencing data yields: