Illumina sequencing platforms generate up to 930 gigabases of high-quality sequence data per lane (NovaSeq X 25B) using a massively parallel short-read sequencing approach. The Illumina systems use sequencing-by-synthesis technology and reversible terminator chemistry. More information describing the technology and the instrument is available from Illumina here. Getting started info: > Register with the GC > Sample Requirements > Sample Submission The DNA Technologies and Expression Analysis Cores of the Genome Center operate one Nextseq 500, and three MiSeqs. We are also sequencing on the NovaSeq X. Steps involved in an Illumina sequencing experiment can be broken down into a series of experimental manipulations, instrument runs, and data analyses. These steps include creation of sequencing libraries, seeding clustering of the flow cell on the sequencer, sequencing by synthesis, and bioinformatics. Please also see the "Beginner's Guide to NGS" information and the video at the bottom of this page, both from Illumina.

Read Length - Data Output

Current average short-read sequencing data yields: