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Getting Started Guide
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To important UC Cores -> Sample preps, 16S, qPCR, Flow Sorting, Sanger, ...
Host Microbe Systems Core -- 16S
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Sanger Sequencing @ UCB
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Flow Cytometry & Sorting
Getting Started
Getting Started Guide
Sample and Library Requirements
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Intro: Illumina Sequencing
Intro: Element Biosciences AVITI Sequencing
Intro: Pacbio Sequencing
Intro: Nanopore Sequencing
Intro: Spatial Biology and Transcriptomics
Services
Short-Read Sequencing Services – Illumina & Element Bio AVITI
DNA Sequencing (whole-genome shotgun, targeted, amplicon, exome, ChIP, reduced-representation, Methyl, …)
RNA-Sequencing (high-throughput mRNA-Seq, total RNA-seq, 3'-Tag-Seq, miRNA-seq, ... )
Gene Expression Profiling with 3' Tag-Seq
Nanopore Sequencing – PromethION
PacBio Revio Library Prep & Sequencing
Linked Read Sequencing – 10X Genomics Chromium Technology
High Molecular Weight DNA Isolation (HMW-DNA)
Illumina & Aviti Sample and Library Requirements
DNA/RNA Quantification & Library QC
DNA and RNA Extraction
Single Cell Expression Profiling & Genomics (10X Genomics, SPLiT-Seq & Plate-based scRNA-Seq)
Spatial Transcriptomics on the Xenium Platform
Spatial Transcriptomics on the Visium HD Platform
Genotyping – Illumina Infinium, Fluidigm EP1
Fluidigm EP1
discontinued - Infinium Genotyping
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Illumina & Aviti Sample and Library Requirements
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Shared Equipment
Booking Shared Instruments - Equipment Calendar
Equipment Training & Use
iconPCR - Adaptive PCR
Agilent Bioanalyzer 2100
FilterMax F5 Plate Reader
Qubit Fluorometer
LabChip GX Touch HT (High-Throughput Bioanalyzer)
Nanodrop Spectrophotometer
Covaris E220 Sonicator
BluePippin and Pippin HT
Robotic Liquid Handlers
Fluidigm Access Array
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