
The mission of the DNA Technologies Core at the UC Davis Genome Center is to provide DNA analytical services to campus and other researchers. We are currently focused on Next-Gen Sequencing (NGS) and genotyping, using mainly Illumina (HiSeq 4000, NextSeq, and MiSeq), Oxford Nanopore PromethION, PacBio Sequel, and Fluidigm (C1-single cell analysis and Access Array) , and 10X Genomics Chromium platforms (linked-read genome sequencing). Our goal is to enable access to high throughput genome-wide analyses at economical recharge rates, as a functional extension of your laboratory.

The mission of the Expression Analysis Core at the UC Davis Genome Center is to provide services and technologies for gene expression analyses at the genomic level. We are exploiting the latest technologies for example for our high-throughput Tag-Seq Gene Expression Profiling services. We offer standard as well as specialized and custom RNA-seq library prep services for Illumina sequencing. The PacBio Iso-Seq protocol enables full-length transcript sequencing and thus the discovery of new gene isoforms. The 10X Genomics Chromium single-cell platform allows single-cell and single-nuclei gene expression profiling for up to 10,000 cells or nuclei. Workshops in these technologies are an important part of facility operations. We also offer access to liquid handlers and nucleic acid analyzers to facilitate your experiments. Read more about Getting Started in the Core.
Services and Equipment
Technology Platforms | Facility Services |
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Illumina Sequencing HiSeq 4000, NextSeq & MiSeq PacBio Sequencing PacBio RSII, Sequel 10X Genomics Chromium Single-Cell Gene Expression; Whole Genome Linked Reads Fluidigm C1 Single Cell Genomics Auto-Prep Fluidigm Access Array Target Amplification Infinium Genotyping Fluidigm EP1 Genotyping | Next-Gen Sequencing (Illumina and PacBio) Next-Gen Library Prep DNA Quantification/Library QC Single Cell Genomics Genotyping Expression Analysis Equipment: Training & Access Sample QC: Agilent Bioanalyzer, Nanodrop, Caliper LabChip GX Sample Prep: Covaris E220, Diagenode Bioruptor & Bioruptor NGS, Sage Science BluePippin, Pippin HT Automated Library Prep: Caliper Sciclone G3, IntegenX Apollo 324, Fluidigm Access Array |