Library quality control (QC) is essential prior to sequencing. Our library preparation service includes Agilent Bioanalyzer or Tapestation QC, and our sequencing service includes qPCR quantification. However, if you are preparing libraries such as PCR-free libraries prior to pooling, we offer a stand-alone qPCR service using the KAPA Biosystems SYBR FAST Library Quantification Kit for Illumina Sequencing Platforms. This kit measures absolute numbers of molecules containing the Illumina adapter sequences, thus providing a highly accurate measurement of amplifiable molecules available for cluster generation.
When analyzing a larger number number of samples or libraries (> 24) the LabChip GX instrument offers a more affordable and faster alternative to the Bioanalyzer.
Quantification of double-stranded DNA using the Qubit Fluorometer (Life Technologies) is also available as a service. If you would like us to pool your libraries we can do this too (pooling is typically based on Qubit measurements, unless the libraries are PCR-free).
Bioanalyzer QC is offered on the DNA High Sensitivity, RNA Nano and RNA Pico chips. Tapestation QC is offered on DNA D1000 (<1500 bp), D5000 (<10,000 bp), Genomic (max 40,000 bp) and RNA tapes. Samples need to be at the medium to upper concentration range for a successful run (additional fees will apply if the sample is not diluted appropriately); please see the 'Sample QC Requirements' at the bottom of this page. For any of these services, please provide at least 2 ul of sample in labelled 0.6 or 1.5 ml tubes. Samples should be accompanied by the QC request form. For prices, please refer to the 'Sample QC' section of the Prices page.