RNA-seq protocols using ribo-depletion can be significantly more tolerant to low RNA integrity samples. Please see this APPLICATION NOTE from KapaBiosystems/Roche for in-depth information.
Please note that the following RNA-seq recommendations are necessarily very generic and simplified. They do apply to common scenarios but might not be right for your project.
Please support us by acknowledging our services in your publications.
We have received NIH funding for the purchase of some of our instruments and this support should be mentioned. Please add a sentence like this to your acknowledgments: “The sequencing was carried out at the DNA Technologies and Expression Analysis Cores at the UC Davis Genome Center (RRID:SCR_017740), supported by NIH Shared Instrumentation Grant 1S10OD010786-01.”
We ask you to mention our services in the acknowledgments of your publications. This will support our applications to instrumentation grants and thus help to improve our services.
Please see the the details here.
If you feel we contributed intellectually to your project it is appropriate for us to share authorship. If you have any questions please talk to us about it.
The building front doors are open in the morning during business hours. After 5 pm please give us a call at 754-9143 and we will come and let you in.
Our usual business hours are weekdays from 9 am to 5 pm.
The BGI@UCDavis was located in the School of Medicine on the Sacramento campus and did provide sequencing services for large scale sequencing projects up until September 2015. This BGI facility is now closed. Since our DNA Technologies Core operates the latest generation of sequencers (HiSeq3000/4000), which offers a 7 times increased throughput compared to the previous generation, our Core is now taking on also large sequencing projects.
Several pieces of equipment are available for use during normal working hours for a nominal ‘instrument use’ fee. To use the Shared Equipment, you will need to first be trained by Core staff (there is a training fee per person).
